fluorescein isothiocyanate fitc conjugated anti human cd14 61d3  (Thermo Fisher)

Journal: Frontiers in Immunology

Article Title: Single-cell RNA-sequencing highlights a curtailed NK cell function in convalescent COVID-19 pregnant women

doi: 10.3389/fimmu.2025.1560391

Figure Lengend Snippet: Validation of immunophenotyping studies and dysregulated humoral immune response in German cohort 2. (a) German Cohort 2: description of cohort and experimental plan using matched PBMCs and serum from pregnant healthy controls (Preg-HC) and COVID-19 recovered (Preg-R) pregnant women. (b) Reduced percentage of CD14 + monocytes and significantly increased CD14 + CD16 + in Preg-R patients. Box plots show median, interquartile range (IQR), and the whiskers corresponding to the highest and lowest points within 1.5 times of IQR. Each dot represents an individual sample. Wilcoxon rank-sum test was used for p-value significance to compare pregnant healthy control (Preg-HC) and COVID-19 recovered (Preg-R). P ≤0.05 considered significant (*p ≤0.05). (c) Box and whisker plot representing the percentage of Naïve, CM, EM I, and EM II CD8 + T cells in Preg-HC and Preg-R patients. CD8 + Naïve and CD8 + CM T cells were lower in Preg-R. CD8 + EM I and CD8 + EM II T cells were significantly increased Preg-R. Wilcoxon rank-sum test was used for p-value significance. P ≤0.05 considered significant (*p ≤0.05). (d) Box and whisker plot representing NKT cells in Preg-R compared with Preg-HC. (e) Box and whisker’s plot showing reduced CD56 + NK cells and CD56 + CD16 + NK II cells in Preg-R. Increased levels of CD56 - HLA-DR + lymphoid cells were significantly increased in Preg-R. Wilcoxon rank-sum test was used for p-value significance. P ≤0.05 considered significant (*p ≤0.05). (f) High levels of Nucleocapsid IgG antibody levels in serum of Preg-R group compared to Preg-HC (left graph). Wilcoxon rank-sum test was used for p-value significance. P ≤0.05 considered significant (*p ≤0.05). Spike S1 (middle graph) and RBD IgG concentration (ug/mL; right graph) at different collection time-points (CT) points (CT1, 0 day; CT2, 39 days; and CT3, 89 days post infection) during their pregnancy. Decreased levels of Spike S1 and RBD IgG antibodies 89 days post first collection time point in Preg-R group. Each dot represents an individual. Kruskal–Wallis test, adjusted for Dunn’s multiple comparisons test. P ≤0.05 considered significant (*p ≤0.05). (g) Examination of IL-10, MCP-1, and IL-8 levels in the serum of recovered women up to 89 days post infection. Each dot represents an individual on a box-whisker plot. CT1, 0 day; CT2, 39 days; and CT3, 89 days post infection-during their pregnancy Kruskal–Wallis test, adjusted for Dunn’s multiple comparisons test. P ≤0.05 considered significant (*p ≤0.05, **p ≤0.01, ***p ≤0.001, ****p ≤0.0001).

Article Snippet: Anti-Human CD14 Alexa FluorTM 700 (clone 61D3) , Thermo Fisher Scientific (eBioscienceTM) , Cat#56-0149-42.

Techniques: Biomarker Discovery, Control, Whisker Assay, Concentration Assay, Infection

Journal: Cell reports

Article Title: Distinct features of a peripheral T helper subset that drives the B cell response in dengue virus infection

doi: 10.1016/j.celrep.2025.115366

Figure Lengend Snippet:

Article Snippet: CD14, APC-eFluor 780, 61D3 , eBioscience , Cat# 47-0149-42; RRID: AB_1834358.

Techniques: Purification, Virus, Recombinant, Lysis, Staining, Electron Microscopy, Enzyme-linked Immunospot, Enzyme-linked Immunosorbent Assay, Amplification, Gene Expression, Software